Basic Information:
Application ID:
:
Sample Classification:
: Chemical Industry
Separation Mode:
: Ion-exchange Chromatography
Product Line:
: Carbohydrate
Method:
:
Chromatographic Conditions:
:
Column:
ReproSil Carbohydrate 5 μm (250x4.6 mm)
Flow Rate: 1.4 ml/min
Eluent: ACN/Water (68/32)
Injection Volume: 10 μl
Temperature: 35°C
Detection: RI
This detailed chromatographic analysis is designed for the separation and identification of carbohydrates in chemical industry samples using ion-exchange chromatography. The method employs a ReproSil Carbohydrate column with a particle size of 5 microns and dimensions of 250 x 4.6 mm, ensuring high resolution and efficiency. The mobile phase consists of acetonitrile and water in a ratio of 68:32, which helps in optimal separation of sugar compounds. The flow rate is set at 1.4 mL/min, and an injection volume of 10 μL is used to maintain consistency and accuracy. The system is operated at a controlled temperature of 35°C, and the detection is carried out using a refractive index detector (RI). This setup is ideal for precise and reliable carbohydrate analysis in various applications.
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