Isoflavone, fructose, glucose, sucrose, maltose, maltotriose

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Basic Information:

Application ID

:

Sample Classification

: Chemical industry

Separation Mode

: Ion-exchange chromatography

Product Line

: Carbohydrate

Method

:

Chromatographic conditions

:

Column: ReproSil Carbohydrate 5 μm (250x4.6 mm)

Flow: 1.4 ml/min

Eluent: ACN/Water (68/32)

Injection: 10 μl

Temp.: 35° C

Det.: RI

This technical document provides detailed information on the separation and analysis of carbohydrate compounds using ion-exchange chromatography. The method is specifically designed for applications in the chemical industry, where precise identification and quantification of carbohydrates are essential. The column used is a high-performance ReproSil Carbohydrate column with a particle size of 5 microns and dimensions of 250 x 4.6 mm. The mobile phase consists of a mixture of acetonitrile and water in a ratio of 68:32. The flow rate is set at 1.4 ml per minute, and the injection volume is 10 microliters. The analysis is carried out at a temperature of 35°C, and a refractive index detector (RI) is used for detection. This setup ensures accurate and reliable results for carbohydrate analysis.